In Vitro Evaluation of Dental Resin Monomers, Triethylene Glycol Dimethacrylate (TEGDMA), and 2-Hydroxyethyl Methacrylate (HEMA) in Primary Human Melanocytes: A Pilot Study

Triethylene glycol dimethacrylate (TEGDMA) and 2-hydroxyethyl methacrylate (HEMA), two resin monomers often used in dental restorative materials, are leached due to insufficient polymerization contact cells of the oral cavity. Despite reports on cytotoxicity these different cells, their effects melanocytes remain unknown. This first report provides novel data impact TEGDMA HEMA melanocyte viability functions by utilizing primary human from lightly pigmented (HEMn-LP) foreskin as a representative model melanocytes. Results show that induced higher than significant morphological alterations (increased dendricity) at lowest concentration 0.25 mM onwards. achieved similar but only an 8-fold (2 mM), while lower concentrations did not induce any change cellular melanin or morphology. suppressed intracellular tyrosinase activity 1 2 mM, had no effect, although none altered directly acellular system. alter ROS production. LPS-induced IL-6 cytokine secretion greater degree HEMA, indicating its capacity dampen immune response. Our findings demonstrate melanocytes, stimulate dendricity impair responses, indicative homeostasis. Furthermore, it is worth considering alternative light exhibited millimolar compared well differentiation function.